Lifeboat Foundation

To show the capability of the OrganoidChip in enabling higher-resolution imaging, we used confocal microscopy for several organoids immobilized on the chip. Representative images show improved optical segmentation and the ability to resolve single cells within an organoid (Fig. 4 d). The co-localized EthD-1-and Hoechst-stained nuclei are resolvable and can potentially be used to increase the accuracy of viability measurements. Future implementation of 3D-segmentation using AI-assisted algorithms in the analysis pipeline can provide more accurate estimations of cellular viability in larger screens.

Next, we measured the effect of DOX treatment on the beating kinetics of cardiac organoids. To do this, we relied on calcium fluorescence imaging, as it has been shown to be a good approximation of the cardiomyocytes’ action potentials³². Calcium imaging proved beneficial for beating and contraction parameters since smaller beating portions cannot necessarily be detected from brightfield images, particularly when organoids have been compromised as a result of drug treatment.

When assessing drug effects, we observed some degree of variability in the spontaneous contractile behaviour and beating kinetics between cardiac organoids. Such variability often skews any averaged parameter value across organoids and does not reflect the effect of the treatment conditions on organoid health. To address this challenge, we tracked each individual organoid’s beating off-and on-chip. The drug-induced functionality results are therefore reported as averages of fractional changes of each individual organoid’s beating kinetics parameters, measured at 48 h post-treatment, on both the chamber slide and on the chip, relative to its pre-treatment value (Eq. 3).

Prior to school getting under way is an ideal time to review your child’s needs for coping with allergies and asthma.

Immunotherapy could be the “shot in the arm” you need to treat your allergies.

ARLINGTON HEIGHTS, Ill. (August 7, 2019) – You may think you can’t tell the difference between the symptoms caused by spring, summer and fall allergies. They all usually involve sneezing, sniffling, itchy eyes and a runny nose. And while symptoms for each Allergies are inappropriate or exaggerated reactions of the immune system to substances that, in the majority of people, cause no symptoms. Symptoms of the allergic diseases may be caused by exposure of the skin to a chemical, of the respiratory system to particles of dust or pollen (or other substances), or of the stomach and intestines to a particular food. rel= tooltip allergy season may be similar, the treatment can look very different, particularly if Immunotherapy is a form of preventive and anti-inflammatory treatment of allergy to substances such as pollens, house dust mites, fungi, and stinging insect venom.

Simply the smell of seafood can make those with an allergy to it violently ill—and therefore more likely to avoid it. The same avoidance behavior is exhibited by people who develop food poisoning after eating a certain meal.

Scientists have long known that the immune system played a key role in our reactions to allergens and pathogens in the environment, but it was unclear whether it played any role in prompting these types of behaviors towards allergic triggers.

According to Yale-led research published July 12 in the journal Nature, it turns out that the immune system plays a crucial role in changing our behaviors.

Researchers developed a new method called wildDISCO that uses standard antibodies to map the entire body of an animal using fluorescent markers. This revolutionary technique provides detailed 3D maps of structures, shedding new light on complex biological systems and diseases. WildDISCO has the potential to transform our understanding of intricate processes in health and disease and paves the way for exciting advancements in medical research. This technology was now introduced in Nature Biotechnology.

In the past, scientists relied on genetically modified animals or specialized labels to make specific structures and cells of interest visible in the entire body of an animal. But these approaches are expensive and time-consuming to create, especially when it comes to body-wide systems such as the nervous system.

A team of scientists from Helmholtz Munich, the LMU University Hospital and the Ludwig-Maximilians Universität München (LMU) now introduced a new method called wildDISCO, which makes use of standard antibodies to map whole bodies of mice. This ultimately enables the creation of detailed three-dimensional maps of normal and diseased structures in mammalian bodies in an easy-to-use and cost-efficient way.

Sports teams spend millions of dollars on their players’ health and fitness and any injuries can be detrimental to their players’ careers. Artificial intelligence (AI) has the potential to significantly change the way that sports spine injuries are diagnosed, treated, and managed. Tools such as Spindle and SpindleX are making it easy to prevent long-term injuries or spinal issues by detecting even the minutest variations in time. However, AI has just begun its foray into the field of healthcare and more importantly radiology or spine imaging.

With AI-related radiology imaging, it is becoming easier to prevent and cure injuries we didn’t even know existed. AI-assisted reports are helping physicians and surgeons take better and more accurate decisions and treatment plans, saving millions of dollars in the healthcare industry. Here are a few examples of how AI is improving the treatment of sports-related spine injuries:

The innate immune system is able to identify foreign invaders and immediately respond to them. This system is important in order to protect the body from harmful substances.

The response to an infection triggers the arrival of cells called neutrophils, which attack the infection, followed by macrophages that attack bacteria and viruses.

Macrophages release cytokines in order to communicate with other cells when they encounter an enemy. Cytokines are small proteins that carry information. The immune system is activated by cytokines, which give the immune cells direction to fight.

Developing Novel DNA-Based Mechano-Technologies For Human Health — Dr. Khalid Salaita, Ph.D. — Emory University

Dr. Khalid Salaita, Ph.D. (https://www.salaitalab.com/salaita) is a Professor of Chemistry at Emory University in Atlanta, Georgia (USA), program faculty in the Department of Biomedical Engineering at Georgia Tech and Emory, program member of Cancer Cell Biology at Winship Cancer Institute, and most recently is the recent winner Future Insight Prize given by Merck KGaA, Darmstadt, Germany (https://www.emdgroup.com/en/research/open-innovation/futurei…aming.html) for his cutting edge work in the area of mechanobiology.

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Michael Shiloh had been studying tuberculosis for about two decades when he started wondering about a seemingly basic question: What makes people with TB cough? This is the disease’s hallmark symptom and a main mode of transmission, but despite training as an infectious disease physician and many years of probing the pathogen as a researcher, Shiloh realized that he didn’t know. A quick search of the literature suggested that “essentially nothing had been studied about it, at least not at the molecular level,” he says.

Elucidating the role of cough in illness means first appreciating its role in health. “Cough is one of these critical defensive processes that we have to clear the respiratory system,” says Stuart Mazzone, a neuroscientist at the University of Melbourne. But it also contributes to disease spread, as research by Shiloh, now at the University of Texas Southwestern Medical Center, and others has described. And dysfunctional control of coughing — resulting in too much coughing or not enough — can cause serious health problems.

Here’s a look at how and why we cough, and some of the ways that coughing can go wrong.