Understanding complex biological and biomedical systems is greatly aided by 3D imaging, which provides much more detailed information than traditional two-dimensional methods. However, live cell and tissue imaging remain challenging due to factors like limited imaging speed and significant scattering in turbid environments.
In this context, multimodal microscopy techniques are notable. Specifically, nonlinear techniques like CRS (coherent Raman scattering) use optical vibrational spectroscopy, providing precise chemical imaging in tissues and cells in a label-free way.
Furthermore, stimulated Raman scattering (SRS) microscopy, a CRS method, can accurately capture images of biomolecules due to the linear relationship between stimulated Raman intensity and the concentration of target molecules. It does so with high sensitivity and without interference from unwanted nonresonant backgrounds.
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